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JoVE Journal Cancer Research
Modelo biomimético tridimensional in vitro de neuroblastoma con andamios basados en colágeno
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Modelo biomimético tridimensional in vitro de neuroblastoma con andamios basados en colágeno

Article DOI: 10.3791/62627-v • 07:48 min • July 9th, 2021
July 9th, 2021 •
*1,2,3, *1,2,3, 4, 3,5,6, 1,2,3,6,7
1Cancer Bioengineering Group, Department of Anatomy and Regenerative Medicine, RCSI University of Medicine and Health Sciences, Dublin, Ireland, 2School of Pharmacy and Biomolecular Sciences, RCSI University of Medicine and Health Sciences, Dublin, Ireland, 3Tissue Engineering Research Group, Department of Anatomy and Regenerative Medicine, RCSI University of Medicine and Health Sciences, Dublin, Ireland, 7Department of Chemistry, Royal College of Surgeons in Ireland (RCSI), 123 St. Stephen's Green, Dublin 2, Ireland, 5Trinity Centre for Bioengineering, Trinity College Dublin, Dublin, Ireland, 6Advanced Materials and Bioengineering Research Centre (AMBER), RCSI and TCD, Dublin, Ireland, 7National Children’s Research Centre, Our Lady's Children's Hospital Crumlin, Dublin, Ireland
* These authors contributed equally

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Este artículo enumera los pasos necesarios para sembrar líneas celulares de neuroblastoma en andamios tridimensionales basados en colágeno descritos anteriormente, mantener el crecimiento celular durante un período de tiempo predeterminado y recuperar andamios para varios análisis de crecimiento y comportamiento celular y aplicaciones posteriores, adaptables para satisfacer una variedad de objetivos experimentales.

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Tridimensional In Vitro Modelo Biomimético Neuroblastoma Andamios a base de colágeno Cáncer pediátrico Tejido tumoral Matriz extracelular (MEC) Progresión de la enfermedad Pronóstico del paciente Respuesta terapéutica Líneas celulares de neuroblastoma Nanohidroxiapatita (nHA) Glicosaminoglicanos (GAG) Hueso y médula ósea Sitios metastásicos Estructura porosa Adhesión celular Proliferación Migración Grupos celulares Terapéutica Situación in vivo Sistema de cultivo basado en andamios Célula bidimensional (2D) Cultivo Cuantificación de ADN Viabilidad Celular
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