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JoVE Journal Cancer Research
Tredimensionell in vitro biomimetisk modell av neuroblastom med hjälp av kollagenbaserade strukturer
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Tredimensionell in vitro biomimetisk modell av neuroblastom med hjälp av kollagenbaserade strukturer

Article DOI: 10.3791/62627-v • 07:48 min • July 9th, 2021
July 9th, 2021 •
*1,2,3, *1,2,3, 4, 3,5,6, 1,2,3,6,7
1Cancer Bioengineering Group, Department of Anatomy and Regenerative Medicine, RCSI University of Medicine and Health Sciences, Dublin, Ireland, 2School of Pharmacy and Biomolecular Sciences, RCSI University of Medicine and Health Sciences, Dublin, Ireland, 3Tissue Engineering Research Group, Department of Anatomy and Regenerative Medicine, RCSI University of Medicine and Health Sciences, Dublin, Ireland, 7Department of Chemistry, Royal College of Surgeons in Ireland (RCSI), 123 St. Stephen's Green, Dublin 2, Ireland, 5Trinity Centre for Bioengineering, Trinity College Dublin, Dublin, Ireland, 6Advanced Materials and Bioengineering Research Centre (AMBER), RCSI and TCD, Dublin, Ireland, 7National Children’s Research Centre, Our Lady's Children's Hospital Crumlin, Dublin, Ireland
* These authors contributed equally

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Denna artikel listar de steg som krävs för att så neuroblastomcellinjer på tidigare beskrivna tredimensionella kollagenbaserade strukturer, upprätthålla celltillväxt under en förutbestämd tidsram och hämta strukturer för flera celltillväxt- och cellbeteendeanalyser och nedströmsapplikationer, anpassningsbara för att tillfredsställa en rad experimentella mål.

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Tredimensionell In vitro biomimetisk modell neuroblastom kollagenbaserade byggnadsställningar barncancer tumörvävnad extracellulär matris (ECM) sjukdomsprogression patientprognos terapeutiskt svar neuroblastomcellinjer nanohydroxiapatit (nHA) glykosaminoglykaner (GAG) ben och benmärg metastaserande platser porös struktur cellfäste proliferation migration cellkluster terapier in vivo-situation ställningsbaserat odlingssystem tvådimensionell (2D) cell Odling DNA-kvantifiering cellviabilitet
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