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Modello biomimetico tridimensionale in vitro di neuroblastoma utilizzando scaffold a base di collagene
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Modello biomimetico tridimensionale in vitro di neuroblastoma utilizzando scaffold a base di collagene

Article DOI: 10.3791/62627-v • 07:48 min • July 9th, 2021
July 9th, 2021 •
*1,2,3, *1,2,3, 4, 3,5,6, 1,2,3,6,7
1Cancer Bioengineering Group, Department of Anatomy and Regenerative Medicine, RCSI University of Medicine and Health Sciences, Dublin, Ireland, 2School of Pharmacy and Biomolecular Sciences, RCSI University of Medicine and Health Sciences, Dublin, Ireland, 3Tissue Engineering Research Group, Department of Anatomy and Regenerative Medicine, RCSI University of Medicine and Health Sciences, Dublin, Ireland, 7Department of Chemistry, Royal College of Surgeons in Ireland (RCSI), 123 St. Stephen's Green, Dublin 2, Ireland, 5Trinity Centre for Bioengineering, Trinity College Dublin, Dublin, Ireland, 6Advanced Materials and Bioengineering Research Centre (AMBER), RCSI and TCD, Dublin, Ireland, 7National Children’s Research Centre, Our Lady's Children's Hospital Crumlin, Dublin, Ireland
* These authors contributed equally

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Questo documento elenca i passaggi necessari per seminare linee cellulari di neuroblastoma su scaffold tridimensionali a base di collagene precedentemente descritti, mantenere la crescita cellulare per un periodo di tempo predeterminato e recuperare scaffold per diverse analisi della crescita cellulare e del comportamento cellulare e applicazioni a valle, adattabili per soddisfare una serie di obiettivi sperimentali.

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Tridimensionale In Vitro Modello biomimetico Neuroblastoma Scaffold a base di collagene Cancro pediatrico Tessuto tumorale Matrice extracellulare (ECM) Progressione della malattia Prognosi del paziente Risposta terapeutica Linee cellulari di neuroblastoma Nanoidrossiapatite (nHA) Glicosaminoglicani (GAG) Osso e midollo osseo Siti metastatici Struttura porosa Attacco cellulare Proliferazione Migrazione Cluster cellulari Terapie Situazione in vivo Sistema di coltura basato su scaffold Cellula bidimensionale (2D) Coltura quantificazione del DNA vitalità cellulare
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