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JoVE Journal Cancer Research
Driedimensionaal in vitro biomimetisch model van neuroblastoom met behulp van op collageen gebaseerde steigers
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Driedimensionaal in vitro biomimetisch model van neuroblastoom met behulp van op collageen gebaseerde steigers

Article DOI: 10.3791/62627-v • 07:48 min • July 9th, 2021
July 9th, 2021 •
*1,2,3, *1,2,3, 4, 3,5,6, 1,2,3,6,7
1Cancer Bioengineering Group, Department of Anatomy and Regenerative Medicine, RCSI University of Medicine and Health Sciences, Dublin, Ireland, 2School of Pharmacy and Biomolecular Sciences, RCSI University of Medicine and Health Sciences, Dublin, Ireland, 3Tissue Engineering Research Group, Department of Anatomy and Regenerative Medicine, RCSI University of Medicine and Health Sciences, Dublin, Ireland, 7Department of Chemistry, Royal College of Surgeons in Ireland (RCSI), 123 St. Stephen's Green, Dublin 2, Ireland, 5Trinity Centre for Bioengineering, Trinity College Dublin, Dublin, Ireland, 6Advanced Materials and Bioengineering Research Centre (AMBER), RCSI and TCD, Dublin, Ireland, 7National Children’s Research Centre, Our Lady's Children's Hospital Crumlin, Dublin, Ireland
* These authors contributed equally

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Dit artikel somt de stappen op die nodig zijn om neuroblastoomcellijnen te zaaien op eerder beschreven driedimensionale op collageen gebaseerde steigers, de celgroei gedurende een vooraf bepaald tijdsbestek te behouden en steigers op te halen voor verschillende celgroei- en celgedragsanalyses en stroomafwaartse toepassingen, aanpasbaar om te voldoen aan een reeks experimentele doelen.

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Driedimensionaal In vitro Biomimetisch model Neuroblastoom Op collageen gebaseerde steigers Kinderkanker Tumorweefsel Extracellulaire matrix (ECM) Ziekteprogressie Prognose van de patiënt Therapeutische respons Neuroblastoomcellijnen Nanohydroxyapatiet (nHA) Glycosaminoglycanen (GAG's) Bot en beenmerg Gemetastaseerde plaatsen Poreuze structuur Celgehechtheid Proliferatie Migratie Celclusters Therapieën In Vivo Situatie Op steigers gebaseerd kweeksysteem Tweedimensionale (2D) cel Kweek DNA-kwantificering levensvatbaarheid van cellen
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