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Evaluation of the In vivo Antitumor Activity of Polyanhydride IL-1α Nanoparticles
JoVE Journal
Cancer Research
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JoVE Journal Cancer Research
Evaluation of the In vivo Antitumor Activity of Polyanhydride IL-1α Nanoparticles
DOI:

09:57 min

June 28, 2021

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Chapters

  • 00:04Introduction
  • 00:55Preparation and Maintenance of HNSCC Cell Line
  • 02:38Tumor Implantation
  • 03:14Blood Collection and Serum Separation
  • 04:20Multiplexing of Collected Serum
  • 04:51Collection of Tumor and Inguinal Lymph Node and Preparation of Single-Cell Suspension
  • 05:58FACS Staining of Single-Cell Suspension
  • 07:42Results: IL-1α Antitumor Activity in HNSCC Mouse Model
  • 09:03Conclusion

Summary

Automatic Translation

A standard protocol is described to study the antitumor activity and associated toxicity of IL-1α in a syngeneic mouse model of HNSCC.

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