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Journal
/
Cancer Research
/
聚酐IL-1α纳米颗粒
体内抗肿瘤
活性评价
JoVE Journal
Cancer Research
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JoVE Journal
Cancer Research
Evaluation of the
In vivo
Antitumor Activity of Polyanhydride IL-1α Nanoparticles
Please note that all translations are automatically generated.
Click here for the English version.
聚酐IL-1α纳米颗粒
体内抗肿瘤
活性评价
DOI:
10.3791/62683-v
•
09:57 min
•
June 28, 2021
•
M. M. Hasibuzzaman
2
,
Kathleen A. Ross
4
,
Aliasger K. Salem
4,5,6
,
Balaji Narasimhan
4
,
Andrean L. Simons
2,3,5,6,7
1
Interdisciplinary Graduate Program in Human Toxicology
,
University of Iowa
,
2
Department of Pathology
,
University of Iowa
,
3
Department of Chemical and Biological Engineering, College of Engineering
,
Iowa State University
,
4
Nanovaccine Institute
,
Iowa State University
,
5
Division of Pharmaceutics and Translational Therapeutics, College of Pharmacy
,
University of Iowa
,
6
Holden Comprehensive Cancer Center
,
University of Iowa
,
7
Department of Oral Pathology, Radiology and Medicine, College of Dentistry
,
University of Iowa
Chapters
00:04
Introduction
00:55
Preparation and Maintenance of HNSCC Cell Line
02:38
Tumor Implantation
03:14
Blood Collection and Serum Separation
04:20
Multiplexing of Collected Serum
04:51
Collection of Tumor and Inguinal Lymph Node and Preparation of Single-Cell Suspension
05:58
FACS Staining of Single-Cell Suspension
07:42
Results: IL-1α Antitumor Activity in HNSCC Mouse Model
09:03
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
描述了一种标准方案来研究HNSCC同系小鼠模型中IL-1α的抗肿瘤活性和相关毒性。
Tags
In Vivo Antitumor Activity
Polyanhydride IL-1α Nanoparticles
Tumor Implantation
Cytokine Analysis
Immune Cell Populations
Antitumor Efficacy
Toxicity Studies
EERL Cells
Cell Culture
Subcutaneous Injection
Submandibular Blood Collection
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