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多活性猪猪Bac转座酶介导的秋粘虫，蜉蝣属果蝇蛆中的种系转化
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JoVE Journal Biology
Hyperactive piggyBac Transposase-mediated Germline Transformation in the Fall Armyworm, Spodoptera frugiperda
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多活性猪猪Bac转座酶介导的秋粘虫，蜉蝣属果蝇蛆中的种系转化

DOI:
10.3791/62714-v

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05:20 min

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September 23, 2021

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,
1Department of Entomology, College of Agriculture, Food and Environment,University of Kentucky

Chapters

  • 00:04Introduction
  • 00:44Microinjection
  • 02:32Fluorescence Screening and Genetic Crossing
  • 03:34Results: Germline Transformation in the Fall Armyworm, Spodoptera frugiperda
  • 04:44Conclusion

Summary

Automatic Translation

Automatic Translation

秋粘虫 Spodoptera frugiperda的种系转化是使用过度活跃的 piggyBac 转座酶的mRNA实现的。

Tags

Fall ArmywormSpodoptera FrugiperdaPiggyBacTransposaseGermline TransformationEGFPMicroinjectionArtificial DietPupaeNeonate LarvaeFluorescence Microscopy

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