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Bestemmelse av trepartsinteraksjon mellom to monomerer av en MADS-boks transkripsjonsfaktor og et kalsiumsensorprotein av BiFC-FRET-FLIM Assay
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Bestemmelse av trepartsinteraksjon mellom to monomerer av en MADS-boks transkripsjonsfaktor og et kalsiumsensorprotein av BiFC-FRET-FLIM Assay

Article DOI: 10.3791/62791-v • 14:34 min • December 25th, 2021
December 25th, 2021 •
*1, *1, 1, 1, 1, 1, 2, 1
1Interdisciplinary Centre for Plant Genomics, Department of Plant Molecular Biology, University of Delhi South Campus, 2University School of Biotechnology, Guru Gobind Singh Indraprastha University
* These authors contributed equally

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Her presenterer vi en metode for å visualisere ternær kompleks formasjon mellom tre proteinpartnere ved hjelp av fluorescerende merkede proteiner av BiFC-basert FRET-FLIM-analyse. Denne metoden er verdifull for å studere proteinproteininteraksjonskomplekser in vivo.

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