JoVE
JoVE
Faculty Resource Center
Research
Behavior
Biochemistry
Biology
Bioengineering
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
JoVE Journal
JoVE Encyclopedia of Experiments
JoVE Chrome Extension
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to your Course
Authors
Librarians
High Schools
About
Sign-In
Sign In
Contact Us
Research
JoVE Journal
JoVE Encyclopedia of Experiments
Education
JoVE Core
JoVE Science Education
JoVE Lab Manual
High Schools
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
JA - 日本語
TR - Türkçe
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
JA - 日本語
TR - Türkçe
Close
Research
Behavior
Biochemistry
Bioengineering
Biology
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
Products
JoVE Journal
JoVE Encyclopedia of Experiments
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
Products
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to Your Course
Teacher Resources
Get in Touch
Instant Trial
Log In
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
JA - 日本語
TR - Türkçe
Journal
/
Biology
/
通过BiFC-FRET-FLIM测定MADS盒转录因子和钙传感器蛋白两种单体之间的三方相互作用
JoVE Journal
Biology
Author Produced
A subscription to JoVE is required to view this content.
Sign in or start your free trial.
JoVE Journal
Biology
Determination of Tripartite Interaction between Two Monomers of a MADS-box Transcription Factor and a Calcium Sensor Protein by BiFC-FRET-FLIM Assay
Please note that all translations are automatically generated.
Click here for the English version.
通过BiFC-FRET-FLIM测定MADS盒转录因子和钙传感器蛋白两种单体之间的三方相互作用
DOI:
10.3791/62791-v
•
14:34 min
•
December 25, 2021
•
Neelima Boora*
1
,
Vibha Verma*
1
,
Ridhi Khurana
,
Gautam Gawande
,
Sanchi Bhimrajka
,
Komal Chaprana
,
Meenu Kapoor
,
Sanjay Kapoor
1
Interdisciplinary Centre for Plant Genomics, Department of Plant Molecular Biology
,
University of Delhi South Campus
,
2
University School of Biotechnology
,
Guru Gobind Singh Indraprastha University
Chapters
00:08
Introduction
01:17
Cloning of Genes in Entry and Destination Vectors
02:01
Growth Conditions for
Nicotiana benthamiana
Plants
03:05
Prepare Bacterial Strains for Agroinfiltration
06:20
Prepare Slides for Fluorescence Visualization
06:45
Fret-Flim Analysis Using a Confocal Laser Scanning Microscope
10:51
Fret-Flim Analysis Using a Confocal Laser Scanning Microscope: The Tripartite Interaction
12:31
Representative Results
13:42
Conclusion
Summary
Automatic Translation
English (Original)
العربية (Arabic)
中文 (Chinese)
Nederlands (Dutch)
français (French)
Deutsch (German)
עברית (Hebrew)
italiano (Italian)
日本語 (Japanese)
한국어 (Korean)
português (Portuguese)
русский (Russian)
español (Spanish)
Türkçe (Turkish)
Automatic Translation
在这里,我们提出了一种通过基于BiFC的FRET-FLIM测定使用荧光标记蛋白可视化三元复合物形成的方法。该方法对于研究
体内
蛋白质 - 蛋白质相互作用复合物很有价值。
Tags
Protein-protein Interactions
BiFC
FRET
FLIM
Tripartite Interaction
MADS-box Transcription Factor
Calcium Sensor Protein
Homodimerization
Nicotiana Benthamiana
Agroinfiltration
Article
Embed
ADD TO PLAYLIST
Usage Statistics
Related Videos
重新用四个转录因子的小鼠胚胎成纤维细胞诱导多能干细胞的产生,强力霉素诱导慢病毒转导系统
双分子荧光互补(附设)检测蛋白质相互作用的洋葱细胞使用的Helios基因枪
使用一种基因编码的钙传感器来监测细胞凋亡过程中线粒体钙水平的动态变化
蛋白膜覆盖含量:一个协议测试可溶性和不溶性蛋白质之间的相互作用
在体外</em
液相亲和捕获试验用磁珠研究蛋白质相互作用:脊髓灰质炎病毒的Nanobody范例
蛋白质纯化方法测定结合亲和力微尺度热泳
电化学发光免疫法测定血清心肌肌球蛋白结合蛋白-C敏感而特异的定量方法
G蛋白偶联受体的基于荧光的钙动员含量表征
蛋白质 - 配体相互作用采用差示扫描荧光法测定
全基因组蛋白质相互作用通过蛋白质片段互补法(PCA)的活细胞筛选
Read Article