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Journal / Biology / Elektron Tomografisi ile Ultrayapısal Olarak…
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JoVE Journal Biology
Elektron Tomografisi ile Ultrayapısal Olarak Korunmuş Kromatinde Görüntüleme Replikasyon Alanları
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Elektron Tomografisi ile Ultrayapısal Olarak Korunmuş Kromatinde Görüntüleme Replikasyon Alanları

Article DOI: 10.3791/62803-v • 14:56 min • May 20th, 2022
May 20th, 2022 •
*1,2, *1,2, *1,3, 1,2, 1, 1, 1, 2, 4, 1,2,5
1Belozersky Institute of Physico-chemical Biology, Lomonosov Moscow State University, 2Faculty of Biology, Lomonosov Moscow State University, 3Faculty of Bioengineering and Bioinformatics, Lomonosov Moscow State University, 4National Research Center, Kurchatov Institute, 5V.I. Kulakov National Medical Research Center for Obstetrics, Gynecology, and Perinatology
* These authors contributed equally

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Bu protokol, yapısal olarak korunmuş kromatin in situ içindeki replikasyon bölgelerinin yüksek çözünürlüklü haritalanması için, önceden gömülmüş EdU-streptavidin-Nanogold etiketleme ve ChromEMT'nin bir kombinasyonunu kullanan bir teknik sunar.

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