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Analyse af oxidativ stress i murintarmorganoider ved hjælp af reaktiv iltartsfølsom fluorogen sonde

Article DOI: 10.3791/62880-v • 09:31 min • September 17th, 2021
September 17th, 2021 •
1,3, 1,4, 1,2,5, 1,6
1Molecular Microbial Pathogenesis Unit, Institut Pasteur, 2Chaire de Microbiologie et Maladies Infectieuses, Collège de France, 3Institut de Biologie Paris Seine (IBPS) - Developmental Biology Unit, Sorbonne Université, CNRS UMR7622, INSERM U1156, 4Molecular Radiotherapy, INSERM U1030, Gustave Roussy, Université Paris-Saclay, 5The Center for Microbes, Development and Health, Institut Pasteur Shanghai and Chinese Academy of Sciences, 6Microenvironment and Immunity Unit, Institut Pasteur

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Den foreliggende protokol beskriver en metode til påvisning af reaktive iltarter (ROS) i tarmmurineorganoiderne ved hjælp af kvalitativ billeddannelse og kvantitative cytometriassays. Dette arbejde kan potentielt udvides til andre fluorescerende sonder for at teste effekten af udvalgte forbindelser på ROS.

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Biologi udgave 175 Murine intestinale organoider ROS-detektion Flowcytometrianalyse Live imaging detection intestinale stamceller ROS-følsomt farvestof oxidativ stress fluorogene sonder
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