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1Molecular Microbial Pathogenesis Unit,Institut Pasteur, 2Chaire de Microbiologie et Maladies Infectieuses,Collège de France, 3Institut de Biologie Paris Seine (IBPS) – Developmental Biology Unit,Sorbonne Université, CNRS UMR7622, INSERM U1156, 4Molecular Radiotherapy, INSERM U1030, Gustave Roussy,Université Paris-Saclay, 5The Center for Microbes, Development and Health,Institut Pasteur Shanghai and Chinese Academy of Sciences, 6Microenvironment and Immunity Unit,Institut Pasteur
Chapters
- 00:05Introduction
- 01:07Intestinal Organoids Culture
- 04:13Visualization of Oxidative Stress in 3D Organoids by Confocal Microscopy
- 05:42Quantification of the Oxidative Stress on the Dissociated Organoids Using Flow Cytometer
- 07:19Results: Detection of ROS in the Intestinal Murine Organoids Using Qualitative Imaging and Quantitative Cytometry Assays
- 08:36Conclusion
Il presente protocollo descrive un metodo per rilevare le specie reattive dell'ossigeno (ROS) negli organoidi murini intestinali utilizzando l'imaging qualitativo e i saggi quantitativi di citometria. Questo lavoro può essere potenzialmente esteso ad altre sonde fluorescenti per testare l'effetto di composti selezionati sui ROS.
