A subscription to JoVE is required to view this content. Sign in or start your free trial.
1Molecular Microbial Pathogenesis Unit,Institut Pasteur, 2Chaire de Microbiologie et Maladies Infectieuses,Collège de France, 3Institut de Biologie Paris Seine (IBPS) – Developmental Biology Unit,Sorbonne Université, CNRS UMR7622, INSERM U1156, 4Molecular Radiotherapy, INSERM U1030, Gustave Roussy,Université Paris-Saclay, 5The Center for Microbes, Development and Health,Institut Pasteur Shanghai and Chinese Academy of Sciences, 6Microenvironment and Immunity Unit,Institut Pasteur
Chapters
- 00:05Introduction
- 01:07Intestinal Organoids Culture
- 04:13Visualization of Oxidative Stress in 3D Organoids by Confocal Microscopy
- 05:42Quantification of the Oxidative Stress on the Dissociated Organoids Using Flow Cytometer
- 07:19Results: Detection of ROS in the Intestinal Murine Organoids Using Qualitative Imaging and Quantitative Cytometry Assays
- 08:36Conclusion
Den nåværende protokollen beskriver en metode for å oppdage reaktive oksygenarter (ROS) i tarm murin organoider ved hjelp av kvalitativ avbildning og kvantitative cytometrianalyser. Dette arbeidet kan potensielt utvides til andre fluorescerende sonder for å teste effekten av utvalgte forbindelser på ROS.
