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土壤样本中昆虫病原真菌的分离与选择及真菌对害虫的毒力评价
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JoVE Journal Biology
Isolation and Selection of Entomopathogenic Fungi from Soil Samples and Evaluation of Fungal Virulence against Insect Pests
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土壤样本中昆虫病原真菌的分离与选择及真菌对害虫的毒力评价

DOI:
10.3791/62882-v

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09:42 min

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September 28, 2021

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, , , , , ,
1Department of Entomology,National Chung Hsing University, 2Department of Agricultural biology,Jeonbuk National University, 3Department of Agricultural Convergence Technology,Jeonbuk National University

Chapters

  • 00:04Introduction
  • 01:04Isolation and Selection of Potential Entomopathogenic Fungi (EPF)
  • 02:271st Pathogenicity Screen and 2nd Virulence Test of Fungal Isolates
  • 03:42Molecular and Morphological Identification of Potential Entomopathogenic Fungi (EPF)
  • 05:23Investigation of Conidial Productivity and Thermotolerance
  • 07:07Effective Conidia Number (ECN) Ranking
  • 07:31Results: Thermotolerance and Conidial Production Data into the ECN Formula and PCA Analysis
  • 08:40Conclusion

Summary

Automatic Translation

Automatic Translation

在这里，我们提出了一种基于黄粉虫（Tenebrio molitor）诱饵系统的方案，该系统用于从土壤样品中分离和选择昆虫致病真菌（EPF）。采用有效的分生孢子数（ECN）公式，根据生理特性选择高耐压EPF，用于野外害虫微生物防治。

Tags

Keywords: Entomopathogenic FungiSoil SamplesInsect PestsIsolationVirulencePathogenicity ScreeningTenebrio MolitorSabouraud Dextrose AgarThermotoleranceConidia Production

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