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JoVE Journal Biology

Tekrarlayan Düşükte Dört Endometriyal İmmün Hücre Tipinin Çok Yönlü Floresan İmmünohistokimyasal Boyanması

Evaluation of Muscle Function of the Extensor Digitorum Longus Muscle Ex vivo and Tibialis Anterior Muscle In situ in Mice

Journal (Biology)

Evaluation of Muscle Function of the Extensor Digitorum Longus Muscle Ex vivo and Tibialis Anterior Muscle In situ in Mice

Determining heat and mechanical pain threshold in inflamed skin of human subjects

Journal (Biology)

Determining heat and mechanical pain threshold in inflamed skin of human subjects

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Tekrarlayan Düşükte Dört Endometriyal İmmün Hücre Tipinin Çok Yönlü Floresan İmmünohistokimyasal Boyanması

Article DOI: 10.3791/62931-v • 05:16 min • August 4th, 2021

August 4th, 2021 •

Yiwei Zhao¹, Gene Chi Wai Man^1,2, Loucia Kit Ying Chan¹, Xi Guo¹, Yingyu Liu³, Tao Zhang¹, Joseph Kwong^1,4, Chi Chiu Wang^1,5, Xiaoyan Chen³, Tin Chiu Li¹

¹Department of Obstetrics and Gynaecology, The Chinese University of Hong Kong, Prince of Wales Hospital, ²Department of Orthopaedics and Traumatology, The Chinese University of Hong Kong, Prince of Wales Hospital, ³Department of Obstetrics and Gynaecology, Shenzhen Baoan Women’s and Children’s Hospital, Shenzhen University, ⁴School of Medicine, Faculty of Medicine and Health Sciences, Keele University, ⁵Li Ka Shing Institute of Health Sciences; School of Biomedical Sciences, The Chinese University of Hong Kong

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Multipleks immünhistokimya ve multispektral görüntülemedeki gelişmelere rağmen, endometriyumda aynı anda majör immün hücrelerin yoğunluğunu ve kümelenişini karakterize etmek bir zorluk olmaya devam etmektedir. Bu makalede, endometriyumdaki dört immün hücre tipinin eşzamanlı lokalizasyonu için ayrıntılı bir multipleks boyama protokolü ve görüntüleme açıklanmaktadır.

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Biyoloji Sayı 174 Multipleks Floresan Bağışıklık hücreleri Yöntemler Boyama

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