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1Department of Obstetrics and Gynaecology,The Chinese University of Hong Kong, Prince of Wales Hospital, 2Department of Orthopaedics and Traumatology,The Chinese University of Hong Kong, Prince of Wales Hospital, 3Department of Obstetrics and Gynaecology, Shenzhen Baoan Women’s and Children’s Hospital,Shenzhen University, 4School of Medicine, Faculty of Medicine and Health Sciences,Keele University, 5Li Ka Shing Institute of Health Sciences; School of Biomedical Sciences,The Chinese University of Hong Kong
Chapters
- 00:05Introduction
- 00:27Multiplex Staining Method
- 02:56Image and Analysis
- 04:09Results: Analysis of the Endometrium Samples with Immunostaining
- 04:49Conclusion
Nonostante i progressi nell'immunoistochimica multiplex e nell'imaging multispettrale, caratterizzare la densità e il raggruppamento delle principali cellule immunitarie contemporaneamente nell'endometrio rimane una sfida. Questo documento descrive un protocollo dettagliato di colorazione multiplex e l'imaging per la localizzazione simultanea di quattro tipi di cellule immunitarie nell'endometrio.
