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JoVE Journal Neuroscience
To peeling metoder for isolering av photoreceptor celle rom i musen netthinnen for protein analyse
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To peeling metoder for isolering av photoreceptor celle rom i musen netthinnen for protein analyse

Article DOI: 10.3791/62977-v • 11:08 min • December 7th, 2021
December 7th, 2021 •
1, 1, 1,2
1Zilkha Neurogenetic Institute, Keck School of Medicine, University of Southern California, 2Department of Cell & Neurobiology, Keck School of Medicine, University of Southern California

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Denne protokollen presenterer to teknikker for å isolere de subcellulære rommene til murine rod fotoreseptorer for proteinanalyse. Den første metoden benytter levende netthinne og cellulosefilterpapir for å skille stang ytre segmenter, mens den andre bruker lyofilisert netthinne og tape for å skrelle bort stang indre og ytre segmentlag.

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