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Digital droplet PCR-metode til kvantificering af AAV Transduktionseffektivitet i Murine Retina
JoVE Journal
Neuroscience
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JoVE Journal Neuroscience
Digital Droplet PCR Method for the Quantification of AAV Transduction Efficiency in Murine Retina
DOI:

09:50 min

December 25, 2021

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Chapters

  • 00:01Introduction
  • 00:57Small Scale AAV Production
  • 02:52Intravitreal Injection of AAV
  • 04:17Fluorescence and Fundus Imaging
  • 04:48Retina Isolation
  • 05:16Tissue Genomic DNA Isolation
  • 05:57Digital Droplet PCR Analysis of Mouse Retina Samples for Quantification of Viral Genomes
  • 07:52Results
  • 09:07Conclusion

Summary

Automatic Translation

Denne protokol præsenterer, hvordan man kvantificerer AAV transduktion effektivitet i musen nethinden ved hjælp af digital dråbe PCR (dd-PCR) sammen med lille skala AAV produktion, intravitreal injektion, retinal billeddannelse, og retinal genomisk DNA isolation.

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