Journal
/
Neuroscience
/
Digital droplet PCR-metode til kvantificering af AAV Transduktionseffektivitet i Murine Retina
JoVE Journal
Neuroscience
Author Produced
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Neuroscience
Digital Droplet PCR Method for the Quantification of AAV Transduction Efficiency in Murine Retina

Digital droplet PCR-metode til kvantificering af AAV Transduktionseffektivitet i Murine Retina

DOI:
10.3791/63038-v

•

09:50 min

•

December 25, 2021

•

, , , ,
1Molecular Biology, Genetics and Bioengineering Program,Sabanci University, 2Nanotechnology Research and Application Center (SUNUM),Sabanci University

Chapters

  • 00:01Introduction
  • 00:57Small Scale AAV Production
  • 02:52Intravitreal Injection of AAV
  • 04:17Fluorescence and Fundus Imaging
  • 04:48Retina Isolation
  • 05:16Tissue Genomic DNA Isolation
  • 05:57Digital Droplet PCR Analysis of Mouse Retina Samples for Quantification of Viral Genomes
  • 07:52Results
  • 09:07Conclusion

Summary

Automatic Translation

Automatic Translation

Denne protokol præsenterer, hvordan man kvantificerer AAV transduktion effektivitet i musen nethinden ved hjælp af digital dråbe PCR (dd-PCR) sammen med lille skala AAV produktion, intravitreal injektion, retinal billeddannelse, og retinal genomisk DNA isolation.

Tags

Digital Droplet PCRAAV TransductionMurine RetinaQuantificationAbsolute QuantificationCRISPRGene TherapyMitochondrial DNATransfectionVirus Purification

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

In vivo Electroporation of Developing Mouse Retina

Vurdering af Vascular Regeneration i CNS Brug af musen Retina

Intracerebroventrikulær Viral Injektion af Neonatal Mouse Hjerne for Vedvarende og Udbredt Neuronal transduktion

Imaging Ca<sup> 2+</sup> Dynamics i Cone Lysmodtageren Axon Terminals i Mouse Retina

Okulær kinematik målt ved In Vitro Stimulation af hjernenerver i skildpadden

En volumetriske metode til kvantificering af Cerebral vasospasme i en Murine Model af subaraknoid blødning

Transpupillær to-foton In vivo Billeddannelse af musens nethinde

To peelingmetoder til isolering af fotoreceptorcellerum i musenehinden til proteinanalyse

In vivo Aflæsninger af vaskulær skade i musens nethinde for at fremme reproducerbarhed

Kvantificering af immunostained Caspase-9 i retinalt væv

Read Article