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Quantification of Immunostained Caspase-9 in Retinal Tissue

Quantification of Immunostained Caspase-9 in Retinal Tissue

JoVE Journal
Neuroscience

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JoVE Journal Neuroscience

Quantification of Immunostained Caspase-9 in Retinal Tissue

Quantification of Immunostained Caspase-9 in Retinal Tissue

1,374 Views

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05:18 min

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July 25, 2022

DOI:

10.3791/64237-v

DOI:

10.3791/64237-v

•

05:18 min

July 25, 2022

•

1363 Views

Crystal K. Colón Ortiz¹, Anna M. Potenski², Kendra V. Johnson¹, Claire W. Chen¹, Scott J. Snipas³, Ying Y. Jean¹, Maria I. Avrutsky*¹, Carol M. Troy*^1,4,5

¹Department of Pathology & Cell Biology, Vagelos College of Physicians and Surgeons,Columbia University ²Department of Molecular Pharmacology and Therapeutics, Vagelos College of Physicians and Surgeons,Columbia University ³NCI-designated Cancer Center,Sanford Burnham Prebys Medical Discovery Institute ⁴Department of Neurology, Vagelos College of Physicians and Surgeons,Columbia University ⁵The Taub Institute for Research on Alzheimer’s Disease and the Aging Brain, Vagelos College of Physicians and Surgeons,Columbia University

Summary

Presented here is a detailed immunohistochemistry protocol to identify, validate, and target functionally relevant caspases in complex tissues.

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