Waiting
Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Neuroscience / Quantification of Immunostained Caspase-9 in…
JoVE Journal
Neuroscience

A subscription to JoVE is required to view this content.

JoVE Journal Neuroscience
Quantification of Immunostained Caspase-9 in Retinal Tissue
Isolation of Distinct Cell Populations from the Developing Cerebellum by Microdissection
Journal (Neuroscience)
Isolation of Distinct Cell Populations from the Developing Cerebellum by Microdissection
Assaying Surface Expression of Chemosensory Receptors in Heterologous Cells
Journal (Neuroscience)
Assaying Surface Expression of Chemosensory Receptors in Heterologous Cells
 

Quantification of Immunostained Caspase-9 in Retinal Tissue

Article DOI: 10.3791/64237-v • 05:18 min • July 25th, 2022
July 25th, 2022 •
1, 2, 1, 1, 3, 1, *1, *1,4,5
1Department of Pathology & Cell Biology, Vagelos College of Physicians and Surgeons, Columbia University, 2Department of Molecular Pharmacology and Therapeutics, Vagelos College of Physicians and Surgeons, Columbia University, 3NCI-designated Cancer Center, Sanford Burnham Prebys Medical Discovery Institute, 4Department of Neurology, Vagelos College of Physicians and Surgeons, Columbia University, 5The Taub Institute for Research on Alzheimer’s Disease and the Aging Brain, Vagelos College of Physicians and Surgeons, Columbia University
* These authors contributed equally

Chapters

Summary
Automatic Translation

Presented here is a detailed immunohistochemistry protocol to identify, validate, and target functionally relevant caspases in complex tissues.

Tags

Immunostain Caspase-9 Retinal Tissue Immunohistochemical Method Cell Biology Therapeutic Targets Confocal Microscopy Stained Retinal Sections Fiji Color Channels Z Stack Max Intensity Projection Brightness And Contrast Interface Pixel Value
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter