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Quantification of Immunostained Caspase-9 in Retinal Tissue
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Neuroscience
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JoVE Journal Neuroscience
Quantification of Immunostained Caspase-9 in Retinal Tissue

Quantification of Immunostained Caspase-9 in Retinal Tissue

DOI:
10.3791/64237-v

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05:18 min

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July 25, 2022

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, , , , , , ,
1Department of Pathology & Cell Biology, Vagelos College of Physicians and Surgeons,Columbia University, 2Department of Molecular Pharmacology and Therapeutics, Vagelos College of Physicians and Surgeons,Columbia University, 3NCI-designated Cancer Center,Sanford Burnham Prebys Medical Discovery Institute, 4Department of Neurology, Vagelos College of Physicians and Surgeons,Columbia University, 5The Taub Institute for Research on Alzheimer’s Disease and the Aging Brain, Vagelos College of Physicians and Surgeons,Columbia University

Chapters

  • 00:05Introduction
  • 00:48Quantification of Caspase Levels
  • 03:21Results: Analyze and Quantify Caspase-9 Levels in the Retinal Tissue
  • 04:34Conclusion

Summary

Automatic Translation

Automatic Translation

Presented here is a detailed immunohistochemistry protocol to identify, validate, and target functionally relevant caspases in complex tissues.

Tags

Keywords: ImmunohistochemistryCaspase-9Confocal MicroscopyQuantificationApoptosisCell BiologyTherapeutic TargetsDiseaseZ StackBrightness And ContrastColocalizationVascular MarkerNeuronal Areas

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