Journal
/
Cancer Research
/
Intratibial Osteosarcoma Cell Injection to Generate Orthotopic Osteosarcoma and Lung Metastasis Mouse Models
JoVE Journal
Cancer Research
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Cancer Research
Intratibial Osteosarcoma Cell Injection to Generate Orthotopic Osteosarcoma and Lung Metastasis Mouse Models

Intratibial Osteosarcoma Cell Injection to Generate Orthotopic Osteosarcoma and Lung Metastasis Mouse Models

DOI:
10.3791/63072-v

•

04:25 min

•

October 28, 2021

•

, , , , ,
1Longhua Hospital,Shanghai University of Traditional Chinese Medicine, 2Key Laboratory of theory and therapy of muscles and bones, Ministry of Education

Chapters

  • 00:04Introduction
  • 00:50Preparation of Cells
  • 01:41Surgery for Orthotopic Injection of the Osteosarcoma Cells
  • 02:53Results: Development of Orthotopic Osteosarcoma and Lung Metastasis in Mouse Models
  • 03:55Conclusion

Summary

Automatic Translation

Automatic Translation

The present protocol describes intratibia osteosarcoma cell injection to generate mouse models bearing orthotopic osteosarcoma and pulmonary metastasis lesions.

Tags

Intratibial InjectionOsteosarcoma CellsOrthotopic OsteosarcomaLung MetastasisMouse ModelCell CountingCell SuspensionBasement Membrane MatrixTibia InjectionCell Injection

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

Establishment of Cancer Stem Cell Cultures from Human Conventional Osteosarcoma

A Portal Vein Injection Model to Study Liver Metastasis of Breast Cancer

Intracarotid Cancer Cell Injection to Produce Mouse Models of Brain Metastasis

Practical Considerations in Studying Metastatic Lung Colonization in Osteosarcoma Using the Pulmonary Metastasis Assay

A Preclinical Mouse Model of Osteosarcoma to Define the Extracellular Vesicle-mediated Communication Between Tumor and Mesenchymal Stem Cells

Modeling Osteosarcoma Using Li-Fraumeni Syndrome Patient-derived Induced Pluripotent Stem Cells

Acellular and Cellular Lung Model to Study Tumor Metastasis

Patient-derived Orthotopic Xenograft Models for Human Urothelial Cell Carcinoma and Colorectal Cancer Tumor Growth and Spontaneous Metastasis

Three-Dimensional Bone Extracellular Matrix Model for Osteosarcoma

Pathological Analysis of Lung Metastasis Following Lateral Tail-Vein Injection of Tumor Cells

Read Article