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Immunology and Infection
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制备珠状支持脂质双分子层研究T细胞免疫突触的颗粒输出
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Immunology and Infection
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JoVE Journal
Immunology and Infection
Preparation of Bead-supported Lipid Bilayers to Study the Particulate Output of T Cell Immune Synapses
Please note that all translations are automatically generated.
Click here for the English version.
制备珠状支持脂质双分子层研究T细胞免疫突触的颗粒输出
DOI:
10.3791/63130-v
•
11:06 min
•
April 01, 2022
•
Pablo F. Céspedes
,
Michael L. Dustin
1
Kennedy Institute of Rheumatology, Nuffield Department of Orthopaedics, Rheumatology and Musculoskeletal Sciences
,
The University of Oxford
Chapters
00:04
Introduction
01:07
Protein Density Calibrations on BSLBs
01:47
Titration of His-Tagged Proteins on 12.5 mol% of DGS-(Ni) NTA-Containing BSLBs
04:02
Measurement of T-cell Output Transfer to BSLBs
08:13
Results: FCM for Absolute Protein Quantification on the Cell Surface
10:17
Conclusion
Summary
Automatic Translation
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在这里,我们提出了使用珠子支持的脂质双分子层逐步重建合成抗原呈递细胞的方案,以及它们用于询问活化T细胞的突触输出。
Tags
Flow Cytometry
Microscopy
Proteomics
RNA Sequencing
Chimeric Antigen Receptors
Signaling Pathways
Genetic Ablation
Protein Calibration
Multicolor Flow Cytometry
Silica Beads
Liposome
Nickel-containing Phospholipids
Argon
Nitrogen
Blocking
BSA
Nickel Sulfate
HBS-HSA Buffer
Protein Dilutions
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