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Journal / Biology / HEK293T Hücrelerinde Bağlanma ve Endositozu…
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HEK293T Hücrelerinde Bağlanma ve Endositozu İzlemek için Kuantum Nokta Konjuge SARS-CoV-2 Spike Trimerlerinin Yüksek Verimli Konfokal Görüntülemesi
A Flow Cytometry-based Assay to Identify Compounds That Disrupt Binding of Fluorescently-labeled CXC Chemokine Ligand 12 to CXC Chemokine Receptor 4
Journal (Biology)
A Flow Cytometry-based Assay to Identify Compounds That Disrupt Binding of Fluorescently-labeled CXC Chemokine Ligand 12 to CXC Chemokine Receptor 4
Building a Better Mosquito: Identifying the Genes Enabling Malaria and Dengue Fever Resistance in A. gambiae and A. aegypti Mosquitoes
Journal (Biology)
Building a Better Mosquito: Identifying the Genes Enabling Malaria and Dengue Fever Resistance in A. gambiae and A. aegypti Mosquitoes
 
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HEK293T Hücrelerinde Bağlanma ve Endositozu İzlemek için Kuantum Nokta Konjuge SARS-CoV-2 Spike Trimerlerinin Yüksek Verimli Konfokal Görüntülemesi

Article DOI: 10.3791/63202-v • 06:39 min • April 21st, 2022
April 21st, 2022 •
1, 2, 2,3, 2, 1
1National Center for Advancing Translational Sciences, 2Optical Sciences Division, Code 5600, Naval Research Laboratory, 3Jacobs Corporation

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Bu protokolde, rekombinant SARS-CoV-2 spike'a konjuge edilen kuantum noktaları, hücre bazlı testlerin, plazma zarındaki hACE2'ye ve ardından bağlı proteinlerin sitoplazmaya endositozunu izlemek için hücre bazlı tahlilleri izlemesini sağlar.

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