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Effektiv Oral RNA Interference (RNAi) Administrasjon til Voksne Anopheles gambiae Mygg
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Effektiv Oral RNA Interference (RNAi) Administrasjon til Voksne Anopheles gambiae Mygg

Article DOI: 10.3791/63266-v • 07:48 min • March 1st, 2022
March 1st, 2022 •
1,2, 1, 3, 4,5, 5,6, 1, 5,7,8
1Division of Parasitic Diseases and Malaria, Entomology Branch, Centers for Disease Control and Prevention, 2Department of Entomology, Cornell University, 3Centro de Estudios en Biotecnologia, Universidad del Valle de Guatemala, 4Department of Cell Biology, Johns Hopkins School of Medicine, 5Johns Hopkins Malaria Research Institute, Johns Hopkins Bloomberg School of Public Health, 6Department of Molecular Microbiology and Immunology, Johns Hopkins Bloomberg School of Public Health and Malaria Research Institute, 7Department of Cell Biology, Johns Hopkins School of Medicine, 8Biomedical Sciences Department, Idaho College of Osteopathic Medicine

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Oral administrering av dsRNA produsert av bakterier, en leveringsmetode for RNA-interferens (RNAi) som rutinemessig brukes i Caenorhabditis elegans, ble vellykket brukt her på voksne mygg. Vår metode muliggjør robuste omvendte genetikkstudier og overføringsblokkerende vektorstudier uten bruk av injeksjon.

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