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Inducción de la inflamación intestinal por transferencia adoptiva de células T CD4+ transgénicas CBir1 TCR a ratones inmunodeficientes
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Inducción de la inflamación intestinal por transferencia adoptiva de células T CD4+ transgénicas CBir1 TCR a ratones inmunodeficientes

Article DOI: 10.3791/63293-v • 07:34 min • December 16th, 2021
December 16th, 2021 •
1, 1, 1
1Department of Microbiology and Immunology, University of Texas Medical Branch

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En este protocolo, se describe un modelo de colitis por transferencia adoptiva de células T específicas de antígeno de la microbiota intestinal. Las células T CD4+ se aíslan de ratones transgénicos CBir1 TCR. Estos son específicos para un antígeno inmunodominante de la microbiota intestinal CBir1 flagelina, que se transfiere a los ratones Rag1-/- receptores, lo que lleva a la inflamación intestinal.

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Medicina Número 178
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