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Induktion af tarmbetændelse ved adoptivoverførsel af CBir1 TCR transgene CD4+ T-celler til immundefekte mus
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Induktion af tarmbetændelse ved adoptivoverførsel af CBir1 TCR transgene CD4+ T-celler til immundefekte mus

Article DOI: 10.3791/63293-v • 07:34 min • December 16th, 2021
December 16th, 2021 •
1, 1, 1
1Department of Microbiology and Immunology, University of Texas Medical Branch

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I denne protokol beskrives en tarmmikrobiota-antigenspecifik T-celleadoptel overførsel colitis-model. CD4+ T-celler isoleres fra CBir1 TCR-transgene mus. Disse er specifikke for et immundominerende tarmmikrobiota-antigen CBir1 flagellin, som overføres til modtager Rag1-/- mus, hvilket fører til tarmbetændelse.

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