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微流体声导术,使用适配子亲和磁珠对革兰氏阴性菌进行流式分离
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Engineering
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JoVE Journal
Engineering
Microfluidic Acoustophoresis for Flowthrough Separation of Gram-Negative Bacteria using Aptamer Affinity Beads
Please note that all translations are automatically generated.
Click here for the English version.
微流体声导术,使用适配子亲和磁珠对革兰氏阴性菌进行流式分离
DOI:
10.3791/63300-v
•
06:08 min
•
October 17, 2022
•
Hong Jin Choi
,
Byung Woo Kim
,
SangWook Lee
,
Ok Chan Jeong
2,4
1
Department of Digital Anti-Aging Health Care
,
Inje University
,
2
Micro device Lab Co., Ltd
,
3
Biohealth Products Research Center (BPRC)
,
Inje University
,
4
Department of Biomedical Engineering
,
Inje University
Chapters
00:04
Introduction
00:45
Microuidic Acoustophoresis Chip Design and Fabrication
01:23
Bacterial Strains and Culture
01:56
Microbeads and Immobilization of Aptamer onto Microbeads
03:10
Acoustophoresis Setup and Operation
04:19
Results: Using Microfluidics to Capture GN Bacteria from Culture Samples at High Speed
05:26
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
本文介绍了使用微流体声传技术以及适配体修饰的微珠的微流控声传导芯片的制造和操作,这些微孔片可用于从培养基中快速,有效地分离革兰氏阴性菌。
Tags
Microfluidic Acoustophoresis
Gram-negative Bacteria Separation
Aptamer Affinity Beads
Flowthrough Separation
Cell Isolation
Microfluidic Device
PZT
Biotinylated DNA Aptamer
Streptavidin-coated Microbeads
Luria-Bertani Medium
Centrifugation
Tris-HCl Buffer
BSA
Article
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