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从土壤中分离可培养酵母和霉菌以研究真菌种群结构
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JoVE Journal
Biology
Isolation of Culturable Yeasts and Molds from Soils to Investigate Fungal Population Structure
Please note that all translations are automatically generated.
Click here for the English version.
从土壤中分离可培养酵母和霉菌以研究真菌种群结构
DOI:
10.3791/63396-v
•
10:33 min
•
May 27, 2022
•
Himeshi Samarasinghe
,
Gregory Korfanty
,
Jianping Xu
1
Department of Biology
,
McMaster University
Chapters
00:04
Introduction
00:50
Incubation of Soil in Liquid Broth and Transferring the Supernatant to the Solid Medium
02:34
Detection of Yeasts, Streaking for Single Colonies, and Identification of Yeast Species
04:04
Isolation of
Aspergillus fumigatus
from Soil
07:32
Results: Isolation and Characterization of Soil Yeast and
Aspergillus fumigatus
Populations
09:37
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
该方案是一种有效,快速的方法,可在短短7天内从大量土壤样品中培养酵母菌和
霉菌烟曲霉
。这些方法可以很容易地修改,以适应实验所需的一系列培养基和温度。
Tags
Soil Samples
Yeast Diversity
Mold Diversity
Fungal Population
Culturable Yeasts
Culturable Molds
Chloramphenicol
Benomyl
Yeast Extract-peptone-dextrose Agar
Roller Drum Incubation
Biosafety Cabinet
Soil Particle Suspension
Colony Selection
Streak Plating
Article
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