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Kerneisolering og multiplexering med lavt input med stregkodede antistoffer af musesympatiske ganglier til enkeltkerne RNA-sekventering
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Kerneisolering og multiplexering med lavt input med stregkodede antistoffer af musesympatiske ganglier til enkeltkerne RNA-sekventering

Article DOI: 10.3791/63397-v • 10:44 min • March 23rd, 2022
March 23rd, 2022 •
1,2, 1, 1,2, 1, 2, 1, 3, 1,2
1Department of Anatomy & Embryology, Leiden University Medical Center, 2Department of Cardiology, Leiden University Medical Center, 3Department of Medical Statistics and Bioinformatics, Leiden University Medical Center

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Denne protokol beskriver den detaljerede prøveforberedelse med lavt input til enkeltkernesekventering, herunder dissektion af musens overlegne cervikale og stellatganglier, celledissociation, kryokonservering, kerneisolering og hashtag-stregkodning.

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