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低输入细胞核分离和多路复用小鼠交感神经节条形码抗体进行单核RNA测序
JoVE Journal
Medicine
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JoVE Journal Medicine
Low-input Nucleus Isolation and Multiplexing with Barcoded Antibodies of Mouse Sympathetic Ganglia for Single-nucleus RNA Sequencing
DOI:

10:44 min

March 23, 2022

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Chapters

  • 00:05Introduction
  • 00:59Dissection of Adult Mouse Superior Cervical Ganglia
  • 02:12Dissection of Adult Mouse Stellate Ganglia
  • 02:57Isolation and Cryopreservation of Mouse Ganglionic Cells
  • 04:56Nucleus Isolation
  • 06:50Nucleus Barcoding with Hashtag Oligos and Multiplexing
  • 08:24Results: Quality Control Analysis of Single-Nucleus cDNA Library Preparation and snRNA-Sequencing
  • 09:55Conclusion

Summary

Automatic Translation

该协议描述了用于单核测序的详细,低输入样品制备,包括小鼠上颈神经节和星状神经节的解剖,细胞解离,冷冻保存,细胞核分离和标签条形码。

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