JoVE
JoVE
Faculty Resource Center
Research
Behavior
Biochemistry
Biology
Bioengineering
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
JoVE Journal
JoVE Encyclopedia of Experiments
JoVE Chrome Extension
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to your Course
Authors
Librarians
High Schools
About
Sign-In
Sign In
Contact Us
Research
JoVE Journal
JoVE Encyclopedia of Experiments
Education
JoVE Core
JoVE Science Education
JoVE Lab Manual
High Schools
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Close
Research
Behavior
Biochemistry
Bioengineering
Biology
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
Products
JoVE Journal
JoVE Encyclopedia of Experiments
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
Products
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to Your Course
Teacher Resources
Get in Touch
Instant Trial
Log In
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Journal
/
Medicine
/
低输入细胞核分离和多路复用小鼠交感神经节条形码抗体进行单核RNA测序
JoVE Journal
Medicine
A subscription to JoVE is required to view this content.
Sign in or start your free trial.
JoVE Journal
Medicine
Low-input Nucleus Isolation and Multiplexing with Barcoded Antibodies of Mouse Sympathetic Ganglia for Single-nucleus RNA Sequencing
Please note that all translations are automatically generated.
Click here for the English version.
低输入细胞核分离和多路复用小鼠交感神经节条形码抗体进行单核RNA测序
DOI:
10.3791/63397-v
•
10:44 min
•
March 23, 2022
•
Yang Ge
2
,
Lieke van Roon
,
H. Sophia Chen
2
,
Ruben Methorst
,
Martin Paton
,
Marco C. DeRuiter
,
Szymon M. Kielbasa
,
Monique R. M. Jongbloed
2
1
Department of Anatomy & Embryology
,
Leiden University Medical Center
,
2
Department of Cardiology
,
Leiden University Medical Center
,
3
Department of Medical Statistics and Bioinformatics
,
Leiden University Medical Center
Chapters
00:05
Introduction
00:59
Dissection of Adult Mouse Superior Cervical Ganglia
02:12
Dissection of Adult Mouse Stellate Ganglia
02:57
Isolation and Cryopreservation of Mouse Ganglionic Cells
04:56
Nucleus Isolation
06:50
Nucleus Barcoding with Hashtag Oligos and Multiplexing
08:24
Results: Quality Control Analysis of Single-Nucleus cDNA Library Preparation and snRNA-Sequencing
09:55
Conclusion
Summary
Automatic Translation
English (Original)
العربية (Arabic)
中文 (Chinese)
Nederlands (Dutch)
français (French)
Deutsch (German)
עברית (Hebrew)
italiano (Italian)
日本語 (Japanese)
한국어 (Korean)
português (Portuguese)
русский (Russian)
español (Spanish)
Türkçe (Turkish)
Automatic Translation
该协议描述了用于单核测序的详细,低输入样品制备,包括小鼠上颈神经节和星状神经节的解剖,细胞解离,冷冻保存,细胞核分离和标签条形码。
Tags
Single-nucleus RNA Sequencing
Cardiac Sympathetic Neurons
Droplet-based Sequencing
Hashtag Oligos
Multiplexing
Superior Cervical Ganglia
Stellate Ganglia
Trypsin Digestion
Article
Embed
ADD TO PLAYLIST
Usage Statistics
Related Videos
一个型号的不安流致动脉粥样硬化小鼠颈动脉部分结扎术和颈动脉内皮细胞的RNA分离的简单方法
测序外周血中细菌菌群:我们的经验与艾滋病毒感染者
收集和唾液DNA提取用于新一代测序
从小鼠癌症模型的循环肿瘤细胞的分离和繁殖
临床样品RNA病毒的无偏深度测序
清醒、无拘无束的小鼠静脉输液同时记录肾交感神经活动和血压的新方法。
念珠菌光滑
全基因组测序检测抗真菌耐药性标志物
代谢综合征心力衰竭模型大鼠心房心肌细胞的分离与保留射血分数的关系
非放射性 l-azidohomoalanine 标记法分离原发性小鼠肝细胞对新生蛋白合成的研究
从人内脏脂肪组织中分离出活的脂肪细胞和基质血管组分,适用于 RNA 分析和巨噬细胞表型分析
Read Article