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使用循环离子淌度光谱仪进行串联离子淌度实验
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Chemistry
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JoVE Journal
Chemistry
Using a Cyclic Ion Mobility Spectrometer for Tandem Ion Mobility Experiments
Please note that all translations are automatically generated.
Click here for the English version.
使用循环离子淌度光谱仪进行串联离子淌度实验
DOI:
10.3791/63451-v
•
08:40 min
•
January 20, 2022
•
Simon Ollivier
2
,
Mathieu Fanuel
2
,
Hélène Rogniaux
2
,
David Ropartz
2
1
INRAE, UR BIA, F-44316 Nantes, France
,
2
INRAE, BIBS Facility, F-44316 Nantes, France
Chapters
00:04
Introduction
01:15
Tuning of the Cyclic IMS Mass Spectrometer
02:39
IMS/IMS-MS Acquisition
06:08
Results: Representative Results for the Cyclic IMS
07:51
Conclusion
Summary
Automatic Translation
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Automatic Translation
离子淌度光谱法(IMS)是质谱法表征生物分子的有趣补充,特别是因为它对异构体敏感。该协议描述了串联IMS(IMS / IMS)实验,该实验允许分离分子并生成其片段的迁移率曲线。
Tags
Cyclic Ion Mobility Spectrometer
Tandem Ion Mobility
Glycan Classification
Molecular Networks
Mass Spectrometry
Ion Mobility
Arrival Time
Calibration
Acquisition Settings
TOF Mode
MSMS Mode
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