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Isolering af mikroRNA'er fra Tick Ex Vivo spytkirtelkulturer og ekstracellulære vesikler
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Isolering af mikroRNA'er fra Tick Ex Vivo spytkirtelkulturer og ekstracellulære vesikler

Article DOI: 10.3791/63618-v • 08:03 min • April 6th, 2022
April 6th, 2022 •
1, 1, 2, 3, 1
1Department of Entomology, Texas A&M University, 2USDA-ARS Cattle Fever Tick Research Laboratory, 3USDA-ARS Veterinary Pest Research Unit

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Den nuværende protokol beskriver isoleringen af mikroRNA'er fra flåtspytkirtler og rensede ekstracellulære vesikler. Dette er en universel procedure, der kombinerer almindeligt anvendte reagenser og forsyninger. Metoden tillader også brugen af et lille antal flåter, hvilket resulterer i mikroRNA'er af høj kvalitet, der let kan sekventeres.

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Biologi udgave 182 ekstracellulære vesikler mikroRNA'er tick-host interface flåter
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