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JoVE Journal
Biochemistry

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An Integrated Workflow of Identification and Quantification on FDR Control-Based Untargeted Metabolome
 

An Integrated Workflow of Identification and Quantification on FDR Control-Based Untargeted Metabolome

Article DOI: 10.3791/63625-v
September 20th, 2022

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Summary September 20th, 2022

We constructed an untargeted metabolomic workflow that integrated XY-Meta and metaX together. In this protocol, we displayed how to use XY-Meta to generate a decoy spectral library from open access spectra reference, and then performed FDR control and used the metaX to quantitate the metabolites after identifying the metabolomics spectra.

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