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准备使用一个不连续Percoll蔗糖密度梯度的从小鼠皮质Synaptoneurosomes

JoVE Journal
Neuroscience

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JoVE Journal Neuroscience

Preparation of Synaptoneurosomes from Mouse Cortex using a Discontinuous Percoll-Sucrose Density Gradient

Please note that all translations are automatically generated. Click here for the English version.

准备使用一个不连续Percoll蔗糖密度梯度的从小鼠皮质Synaptoneurosomes

DOI:

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08:30 min

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September 17, 2011

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Pamela R. Westmark, Cara J. Westmark, Athavi Jeevananthan, James S. Malter

¹Department of Pathology and Laboratory Medicine, Waisman Center for Developmental Disabilities,University of Wisconsin, ²Department of Biochemistry, Waisman Center for Developmental Disabilities,University of Wisconsin

Chapters

00:05Title
01:23Gradient Preparation
02:20Mouse Dissection
03:27Preparation of Homogenate and Synaptoneurosomes
05:51Results: Synaptoneurosome Using a Discontinuous Percoll-sucrose Density Gradient
08:03Conclusion

Summary

Automatic Translation

一个准备从小鼠大脑皮质翻译活跃，完好synaptoneurosomes（SNS）的方法来描述。该方法采用一个不连续Percoll蔗糖密度梯度，允许快速制备活跃的SNS。

Tags

Synaptoneurosomes Mouse Cortex Discontinuous Percoll-Sucrose Density Gradient Homogenization Fractionation Resealed Vesicles Isolated Terminals Synaptic Transmission Pre- And Postsynaptic Characteristics Neuronal Signaling Neurotransmitters Ficoll Cytotoxic Centrifugation Filtration Mechanical Damage Percoll-sucrose Gradient Method Translationally Active SNs Isotonic Conditions

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