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Hydrogel Arrays Enable Increased Throughput for Screening Effects of Matrix Components and Therapeutics in 3D Tumor Models
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Bioengineering
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JoVE Journal Bioengineering
Hydrogel Arrays Enable Increased Throughput for Screening Effects of Matrix Components and Therapeutics in 3D Tumor Models

Hydrogel Arrays Enable Increased Throughput for Screening Effects of Matrix Components and Therapeutics in 3D Tumor Models

DOI:
10.3791/63791-v

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10:49 min

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June 16, 2022

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, , , , , , , , , , , , , , , ,
1University of California Los Angeles

Chapters

  • 00:05Introduction
  • 00:50Preparation of Hydrogel Solution
  • 02:32Illumination and Photocrosslinking of Hydrogels via an LED Array
  • 04:20Setting Up and Drug Treatment of 3D, Matrix-Embedded Cultures
  • 08:22Results: Effect of HA Based 3D Miniaturized Cultures on Matrix Stiffness and Integrin-Binding Peptides
  • 10:24Conclusion

Summary

Automatic Translation

Automatic Translation

The present protocol describes an experimental platform to assess the effects of mechanical and biochemical cues on chemotherapeutic responses of patient-derived glioblastoma cells in 3D matrix-mimetic cultures using a custom-made UV illumination device facilitating high-throughput photocrosslinking of hydrogels with tunable mechanical features.

Tags

Keywords: 3D Tumor ModelsHydrogel ArraysHigh-throughput ScreeningMatrix ComponentsTherapeuticsGBM CellsDrug ResistanceHEPES-buffered SolutionThiolated Hyaluronic AcidPEG NorbornenePEG ThiolCysteine-thiol-containing PeptidesUV LED Illumination

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