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Preparazione di proteomica a cella singola per analisi di spettrometria di massa mediante lisi a calore freddo e un vettore isobarico
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Preparazione di proteomica a cella singola per analisi di spettrometria di massa mediante lisi a calore freddo e un vettore isobarico

Article DOI: 10.3791/63802-v • 06:13 min • December 9th, 2022
December 9th, 2022 •
*1, 1,2,3, *1
1Department of Bioengineering, Northeastern University, 2Barnett Institute, Northeastern University, 3Department of Biology, Northeastern University
* These authors contributed equally

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In questo protocollo, descriviamo come preparare le cellule di mammifero per l'analisi proteomica a singola cellula tramite spettrometria di massa utilizzando reagenti e apparecchiature disponibili in commercio, con opzioni per il pipettaggio manuale e automatico.

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Biologia Numero 190
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