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使用冻热裂解和同量异位载体进行质谱分析的单细胞蛋白质组学制备
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Biology
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JoVE Journal
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Single-Cell Proteomics Preparation for Mass Spectrometry Analysis Using Freeze-Heat Lysis and an Isobaric Carrier
Please note that all translations are automatically generated.
Click here for the English version.
使用冻热裂解和同量异位载体进行质谱分析的单细胞蛋白质组学制备
DOI:
10.3791/63802-v
•
06:13 min
•
December 09, 2022
•
Aleksandra A. Petelski*
1
,
Nikolai Slavov
2,3
,
Harrison Specht*
1
1
Department of Bioengineering
,
Northeastern University
,
2
Barnett Institute
,
Northeastern University
,
3
Department of Biology
,
Northeastern University
Chapters
00:05
Introduction
00:47
Single-Cell Proteomics Sample Preparation
04:43
Results: Single-Cell Proteomics Analysis
05:27
Conclusion
Summary
Automatic Translation
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Automatic Translation
在该协议中,我们描述了如何使用市售试剂和设备通过质谱
法制备
哺乳动物细胞以进行单细胞蛋白质组学分析,并提供手动和自动移液选项。
Tags
Single-cell Proteomics
SCoPE2
Mass Spectrometry
Freeze-heat Lysis
Isobaric Carrier
Trypsin Digestion
Tandem Mass Tagging
LC-MS Analysis
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