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离体 小鼠尿路上皮细胞腺病毒-Cre介导的基因缺失的类器官模型
JoVE Journal
Cancer Research
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JoVE Journal Cancer Research
Ex Vivo Organoid Model of Adenovirus-Cre Mediated Gene Deletions in Mouse Urothelial Cells
DOI:

09:16 min

May 05, 2022

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Chapters

  • 00:04Introduction
  • 00:45Dissection of Mouse Bladder
  • 02:09Dissociation of Urothelial Cells
  • 04:41Counting and Resuspending Cells
  • 05:27Adenoviral Transduction and Plating Organoids
  • 07:06Results: Characterization of Triple Knockout Organoids via Ex Vivo Adenovirus-Cre Recombinase
  • 08:31Conclusion

Summary

Automatic Translation

该协议描述了小鼠尿路上皮类器官的产生和表征过程,这些类器官在感兴趣的基因中具有缺失。这些方法包括收获小鼠尿路上皮细胞,用腺病毒用CMV启动子驱动Cre表达的 离体 转导,以及 体外 体内 表征。

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