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Cancer Research

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离体 小鼠尿路上皮细胞腺病毒-Cre介导的基因缺失的类器官模型
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离体 小鼠尿路上皮细胞腺病毒-Cre介导的基因缺失的类器官模型

Article DOI: 10.3791/63855-v 09:16 min
May 5th, 2022


Summary May 5th, 2022

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该协议描述了小鼠尿路上皮类器官的产生和表征过程,这些类器官在感兴趣的基因中具有缺失。这些方法包括收获小鼠尿路上皮细胞,用腺病毒用CMV启动子驱动Cre表达的 离体 转导,以及 体外 体内 表征。


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