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JoVE Journal Cancer Research
Ex Vivo Organoidmodell av adenovirus-kreiterade genraderingar i musurotelceller
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Ex Vivo Organoidmodell av adenovirus-kreiterade genraderingar i musurotelceller

Article DOI: 10.3791/63855-v • 09:16 min • May 5th, 2022
May 5th, 2022 •
1, 1, 1, 2, 2, 2, 1,2
1Department of Urology, Roswell Park Comprehensive Cancer Center, 2Department of Pharmacology and Therapeutics, Roswell Park Comprehensive Cancer Center

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Detta protokoll beskriver processen för generering och karakterisering av musuroteliala organoider som hyser deletioner i gener av intresse. Metoderna inkluderar skörd av musurotelceller, ex vivo-transduktion med adenovirus som driver Cre-uttryck med en CMV-promotor och in vitro såväl som in vivo-karakterisering .

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Cancerforskning nummer 183
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