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Developmental Biology

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从人多能干细胞中产生气道上皮细胞气液界面培养物

JoVE Journal
Developmental Biology

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JoVE Journal Developmental Biology

Generation of Airway Epithelial Cell Air-Liquid Interface Cultures from Human Pluripotent Stem Cells

Please note that all translations are automatically generated. Click here for the English version.

从人多能干细胞中产生气道上皮细胞气液界面培养物

DOI:

10.3791/63882-v

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10:46 min

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June 14, 2022

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Andrew Berical², Mary Lou Beermann, Shingo Suzuki, Jake LeSuer, Taylor Matte, Brian Davis, Darrell Kotton², Finn Hawkins²

¹Pulmonary Center,Boston University School of Medicine, ²Center for Regenerative Medicine,Boston University and Boston Medical Center, ³Center for Stem Cell and Regenerative Medicine, Brown Foundation Institute of Molecular Medicine,University of Texas Health Science Center

Chapters

00:05Introduction
00:40Thawing of Cryopreserved iPSC-Derived Airway Basal Cells (iBCs)
02:43Dissociation of Spheroids and Expansion of iBCs in 3-D Culture
04:55Evaluation and Purification of NGFR and iBCs
06:10Generation of Mucociliary Air-Liquid Interface (ALI) Cultures
08:37Cryopreservation of iBCs (Optional but Recommended)
09:15Results: Induced Pluripotent Stem Cells for the Derivation of iBCs and ALI Cultures
10:16Conclusion

Summary

Automatic Translation

人类诱导多能干细胞分化方案的最新进展允许逐步衍生器官特异性细胞类型。在这里，我们提供了在气液界面培养物中维持和扩增iPSC衍生的气道基底细胞及其分化为粘纤毛上皮的详细步骤。

Tags

Keywords: Airway Epithelial Cells Air-liquid Interface Human Pluripotent Stem Cells 3D Matrix Cell Culture Cell Differentiation Basal Cell Medium Dispase II Dissociation

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