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Generation of 3D Skin Organoid from Cord Blood-derived Induced Pluripotent Stem Cells
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JoVE Journal Developmental Biology
Generation of 3D Skin Organoid from Cord Blood-derived Induced Pluripotent Stem Cells

Generation of 3D Skin Organoid from Cord Blood-derived Induced Pluripotent Stem Cells

DOI:
10.3791/59297-v

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09:54 min

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April 18, 2019

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1CiSTEM Laboratory, Catholic Induced Pluripotent Stem Cell (iPSC) Research Center, College of Medicine,The Catholic University of Korea, 2Department of Biomedicine & Health Science, Seoul St. Mary’s Hospital, College of Medicine,The Catholic University of Korea, 3Division of Rheumatology, Department of Internal Medicine, Seoul St. Mary’s Hospital, College of Medicine,The Catholic University of Korea

Chapters

  • 00:04Title
  • 00:39Embryonic Body (EB) Generation
  • 02:39Cord Blood Mononuclear Cell-derived-induced Pluripotent Stem Cell (CBMC-iPSC) Keratinocyte Differentiation
  • 04:17CBMC-iPSC Fibroblast Differentiation
  • 05:503D Skin Organoid Generation
  • 08:00Results: Representative CBMC-iPSC-derived 3D Skin Organoid Formation
  • 09:10Conclusion

Summary

Automatic Translation

Automatic Translation

We propose a protocol that shows how to differentiate induced pluripotent stem cell-derived keratinocytes and fibroblasts and generate a 3D skin organoid, using these keratinocytes and fibroblasts. This protocol contains an additional step of generating a humanized mice model. The technique presented here will improve dermatologic research.

Tags

Keywords: 3D Skin OrganoidCord Blood-derived Induced Pluripotent Stem CellsCBMC-iPSCsKeratinocyte DifferentiationFibroblastsEmbryonic Body FormationBone Morphogenetic Protein 4Rho-associated Kinase InhibitorRegenerative MedicineVitronectinEDTAE8 MediumKDM1 Medium

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