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ヒト多能性幹細胞からの気道上皮細胞気液界面培養物の生成
JoVE Journal
Developmental Biology
This content is Free Access.
JoVE Journal Developmental Biology
Generation of Airway Epithelial Cell Air-Liquid Interface Cultures from Human Pluripotent Stem Cells
DOI:

10:46 min

June 14, 2022

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Chapters

  • 00:05Introduction
  • 00:40Thawing of Cryopreserved iPSC-Derived Airway Basal Cells (iBCs)
  • 02:43Dissociation of Spheroids and Expansion of iBCs in 3-D Culture
  • 04:55Evaluation and Purification of NGFR and iBCs
  • 06:10Generation of Mucociliary Air-Liquid Interface (ALI) Cultures
  • 08:37Cryopreservation of iBCs (Optional but Recommended)
  • 09:15Results: Induced Pluripotent Stem Cells for the Derivation of iBCs and ALI Cultures
  • 10:16Conclusion

Summary

Automatic Translation

ヒト人工多能性幹細胞分化プロトコールにおける最近の進歩は、器官特異的細胞型の段階的な誘導を可能にする。ここでは、気液界面培養におけるiPSC由来気道基底細胞の維持・拡大と粘液繊毛上皮への分化のための詳細な手順を述べる。

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