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JoVE Journal Developmental Biology
Generering af luftvejsepitelcelle luft-væske-grænsefladekulturer fra humane pluripotente stamceller
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Generering af luftvejsepitelcelle luft-væske-grænsefladekulturer fra humane pluripotente stamceller

Article DOI: 10.3791/63882-v • 10:46 min • June 14th, 2022
June 14th, 2022 •
1,2, 2, 3, 2, 2, 3, 1,2, 1,2
1Pulmonary Center, Boston University School of Medicine, 2Center for Regenerative Medicine, Boston University and Boston Medical Center, 3Center for Stem Cell and Regenerative Medicine, Brown Foundation Institute of Molecular Medicine, University of Texas Health Science Center

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Nylige fremskridt inden for humane inducerede pluripotente stamcelledifferentieringsprotokoller giver mulighed for trinvis afledning af organspecifikke celletyper. Her giver vi detaljerede trin til vedligeholdelse og udvidelse af iPSC-afledte luftvejsbasalceller og deres differentiering til et mucociliært epitel i luft-væske-grænsefladekulturer.

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Udviklingsbiologi udgave 184
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