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Generering af luftvejsepitelcelle luft-væske-grænsefladekulturer fra humane pluripotente stamceller
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Chapters
- 00:05Introduction
- 00:40Thawing of Cryopreserved iPSC-Derived Airway Basal Cells (iBCs)
- 02:43Dissociation of Spheroids and Expansion of iBCs in 3-D Culture
- 04:55Evaluation and Purification of NGFR and iBCs
- 06:10Generation of Mucociliary Air-Liquid Interface (ALI) Cultures
- 08:37Cryopreservation of iBCs (Optional but Recommended)
- 09:15Results: Induced Pluripotent Stem Cells for the Derivation of iBCs and ALI Cultures
- 10:16Conclusion
Nylige fremskridt inden for humane inducerede pluripotente stamcelledifferentieringsprotokoller giver mulighed for trinvis afledning af organspecifikke celletyper. Her giver vi detaljerede trin til vedligeholdelse og udvidelse af iPSC-afledte luftvejsbasalceller og deres differentiering til et mucociliært epitel i luft-væske-grænsefladekulturer.
