/
/
Generering av Airway Epitelial Cell Air-Liquid Interface Kulturer fra Human Pluripotent Stamceller
This content is Free Access.
Chapters
- 00:05Introduction
- 00:40Thawing of Cryopreserved iPSC-Derived Airway Basal Cells (iBCs)
- 02:43Dissociation of Spheroids and Expansion of iBCs in 3-D Culture
- 04:55Evaluation and Purification of NGFR and iBCs
- 06:10Generation of Mucociliary Air-Liquid Interface (ALI) Cultures
- 08:37Cryopreservation of iBCs (Optional but Recommended)
- 09:15Results: Induced Pluripotent Stem Cells for the Derivation of iBCs and ALI Cultures
- 10:16Conclusion
Nylige fremskritt i menneskeskapte pluripotente stamcelledifferensieringsprotokoller tillater trinnvis avledning av organspesifikke celletyper. Her gir vi detaljerte trinn for vedlikehold og utvidelse av iPSC-avledede luftveisbasalceller og deres differensiering i et mucociliary epitel i luft-væske grensesnittkulturer.
