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Bioengineering
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通过电容式免疫探
针在猪
心中对神经肽动力学进行时间分辨体内测量
JoVE Journal
Bioengineering
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JoVE Journal
Bioengineering
Time-Resolved
In Vivo
Measurement of Neuropeptide Dynamics by Capacitive Immunoprobe in Porcine Heart
Please note that all translations are automatically generated.
Click here for the English version.
通过电容式免疫探
针在猪
心中对神经肽动力学进行时间分辨体内测量
DOI:
10.3791/63926-v
•
08:20 min
•
May 19, 2022
•
Nicholas Kluge
,
Shyue-An Chan
,
Jeffrey L. Ardell
3
,
Corey Smith
1
Department of Physiology and Biophysics
,
Case Western Reserve University
,
2
University of California Los Angeles (UCLA) Cardiac Arrhythmia Center
,
David Geffen School of Medicine
,
3
UCLA Neurocardiology Research Program of Excellence
,
UCLA
Chapters
00:04
Introduction
00:27
Capacitive Immunoprobe Fabrication and Functionalization
03:24
Experimental Setup for In Vitro Detection and Measurement of Peptide
04:41
Adaptation of the Capacitive Immunoprobe for In Vivo Use
05:56
Results: Assessment of Capacitive Immunoprobe
Summary
Automatic Translation
English (Original)
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Automatic Translation
已建立的免疫化学方法
在体内
测量肽递质,依靠微透析或体液抽取来获得样品进行离线分析。但是,这些都受到时空限制。本方案描述了克服现有技术局限性的电容式免疫探针生物传感器的制造和应用。
Tags
Time-resolved Measurement
Neuropeptide Dynamics
Capacitive Immunoprobe
Porcine Heart
In Vivo
Platinum Wire
Dopamine
Poly-dopamine Deposition
Antibody Solution
Flow Chamber
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