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Quantifying Cytoskeleton Dynamics Using Differential Dynamic Microscopy
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Quantifying Cytoskeleton Dynamics Using Differential Dynamic Microscopy

Article DOI: 10.3791/63931-v • 06:37 min • June 15th, 2022
June 15th, 2022 •
1, 2, 2, 3, 3, 2, 2
1Cell Biology, Neurobiology and Biophysics, Department of Biology, Faculty of Science, Utrecht University, 2Department of Physics and Biophysics, University of San Diego, 3Department of Bionanoscience, Kavli Institute of Nanoscience Delft, Delft University of Technology

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Differential dynamic microscopy (DDM) combines features of dynamic light scattering and microscopy. Here, the process of using DDM to characterize reconstituted cytoskeleton networks by quantifying the subdiffusive and caged dynamics of particles in vimentin networks and the ballistic motion of active myosin-driven actin-microtubule composites is presented.

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Quantifying Cytoskeleton Dynamics Differential Dynamic Microscopy Protocol Dynamics Optical Microscopy Techniques Reconstituted Cytoskeleton Networks Software Package Well-documented Example Analysis Files Adaptability Soft Materials Biologically Relevant Materials Image Sequences Microscope Control Software PyDDM Code Repository Parameter File EML File Data Directory File Name Metadata Section Pixel Size Frame Rate Analysis Parameter Section DDM Matrix Calculation Lag Times Fitting Parameters
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