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In Vitro Reconstitution of the Actin Cytoskeleton Inside Giant Unilamellar Vesicles
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Chapters
- 00:04Introduction
- 00:50Preparation of Buffers and Proteins Solutions
- 03:07Preparation of Liposomes Based on the Inverted Emulsion Techniques
- 05:51Microscopy Observation
- 08:11Results: Microscopic Images of the Monolayer Lipid Droplet and Encapsulation of Actin Networks Inside Liposomes
- 09:27Conclusion
In this manuscript, we demonstrate the experimental techniques to encapsulate the F-actin cytoskeleton into giant unilamellar lipid vesicles (also called liposomes), and the method to form a cortex-biomimicking F-actin layer at the inner leaflet of the liposome membrane.
