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JoVE Journal
Bioengineering

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In Vitro Reconstitution of the Actin Cytoskeleton Inside Giant Unilamellar Vesicles
 

In Vitro Reconstitution of the Actin Cytoskeleton Inside Giant Unilamellar Vesicles

Article DOI: 10.3791/64026-v
August 25th, 2022

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Summary August 25th, 2022

In this manuscript, we demonstrate the experimental techniques to encapsulate the F-actin cytoskeleton into giant unilamellar lipid vesicles (also called liposomes), and the method to form a cortex-biomimicking F-actin layer at the inner leaflet of the liposome membrane.

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