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Characterizing RNA Modifications in Single Neurons Using Mass Spectrometry
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Chemistry
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JoVE Journal Chemistry
Characterizing RNA Modifications in Single Neurons Using Mass Spectrometry

Characterizing RNA Modifications in Single Neurons Using Mass Spectrometry

DOI:
10.3791/63940-v

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08:45 min

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April 21, 2022

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, ,
1Beckman Institute for Advanced Science and Technology,University of Illinois Urbana-Champaign, 2Department of Chemistry,University of Illinois Urbana-Champaign

Chapters

  • 00:05Introduction
  • 01:01Isolation of a Single Neuron
  • 03:16Cell Lysis and RNA Digestion for SNRMA-MS
  • 04:29Liquid Chromatography-Tandem Mass Spectrometry
  • 06:15Results: Characterization of Numerous RNA Modifications in Single Neurons
  • 08:08Conclusion

Summary

Automatic Translation

Automatic Translation

Post-transcriptional modifications of RNA represent an understudied layer of translation regulation that has recently been linked to central nervous system plasticity. Here, sample preparation and liquid chromatography-tandem mass spectrometry approach is described for simultaneous characterization of numerous RNA modifications in single neurons.

Tags

Keywords: RNA ModificationsSingle NeuronsMass SpectrometryPost-transcriptional RegulationAplysia CalifornicaGanglia IsolationProtease TreatmentNeuron IsolationArtificial SeawaterMass Spectrometry Analysis

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