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Visualizing the Morphological Characteristics of Neuromuscular Junction in Rat Medial Gastrocnemius Muscle
JoVE Journal
Neuroscience
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JoVE Journal Neuroscience
Visualizing the Morphological Characteristics of Neuromuscular Junction in Rat Medial Gastrocnemius Muscle
DOI:

08:42 min

May 17, 2022

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Chapters

  • 00:04Introduction
  • 00:40Perfusion
  • 01:34Regional Anatomy and Cryosectioning of the Muscle
  • 02:55Multiple Fluorescent Staining with Neurofilament 200 (NF200), Vesicular Acetylcholine Transporter (VAChT), Alpha‐Bungarotoxin (α‐BTX), and Phalloidin (Pha)
  • 04:55Observation and Analyses
  • 06:40Results: Investigating Spatial Correlation of Neuromuscular Junction in Rat Medial Gastrocnemius Muscle
  • 08:13Conclusion

Summary

Automatic Translation

The protocol shows a method to examine spatial correlation among the pre-synaptic terminals, post-synaptic receptors, and peri-synaptic Schwann cells in the rat medial gastrocnemius muscle using fluorescent immunohistochemistry with different biomarkers, namely, neurofilament 200, vesicular acetylcholine transporter, alpha-bungarotoxin, and S100.

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