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Visualisering af de morfologiske egenskaber ved neuromuskulært kryds i rotte medial gastrocnemius muskel
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Chapters
- 00:04Introduction
- 00:40Perfusion
- 01:34Regional Anatomy and Cryosectioning of the Muscle
- 02:55Multiple Fluorescent Staining with Neurofilament 200 (NF200), Vesicular Acetylcholine Transporter (VAChT), Alpha‐Bungarotoxin (α‐BTX), and Phalloidin (Pha)
- 04:55Observation and Analyses
- 06:40Results: Investigating Spatial Correlation of Neuromuscular Junction in Rat Medial Gastrocnemius Muscle
- 08:13Conclusion
Protokollen viser en metode til at undersøge rumlig korrelation mellem de præsynaptiske terminaler, postsynaptiske receptorer og perisynaptiske Schwann-celler i rottemediale gastrocnemiusmuskel ved hjælp af fluorescerende immunohistokemi med forskellige biomarkører, nemlig neurofilament 200, vesikulær acetylcholintransportør, alfa-bungarotoxin og S100.
Tags
Neuromuscular JunctionMedial Gastrocnemius MuscleRatMorphological CharacteristicsSchwann CellsPre-synapticPost-synapticImmunofluorescencePerfusionCryoprotectionCryosectioningTriton X-100Normal Donkey SerumNeurofilament 200Vesicular Acetylcholine TransporterDonkey Anti-rabbit AF 488Donkey Anti-goat AF 594
