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3D原子力組織の変化を研究するために3D保存状態間期核に組み合わさ免疫やDNA FISH
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Combined Immunofluorescence and DNA FISH on 3D-preserved Interphase Nuclei to Study Changes in 3D Nuclear Organization

3D原子力組織の変化を研究するために3D保存状態間期核に組み合わさ免疫やDNA FISH

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18,137 Views

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13:55 min

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February 03, 2013

DOI:

10.3791/50087-v

DOI:
10.3791/50087-v

•

13:55 min
February 03, 2013

•

4 Views
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1Department of Pathology,New York University School of Medicine, 2New York University Center for Health Informatics and Bioinformatics, 3NYU Cancer Institute, 4Department of Pathology and Yale Cancer Center,Yale University School of Medicine

Summary

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ここでは、3D顕微鏡や分析（3D免疫DNA FISH）が続いたDNA FISHによる蛍光抗体法およびDNA配列によるヒストン修飾の同時検出のためのプロトコルを記述します。

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Cite this Article

Chaumeil, J., Micsinai, M., Skok, J. A. Combined Immunofluorescence and DNA FISH on 3D-preserved Interphase Nuclei to Study Changes in 3D Nuclear Organization. J. Vis. Exp. (72), e50087, doi:10.3791/50087 (2013).

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